Through the elimination diet and challenges we found out that annatto 160b causes a severe reaction in our 7 year old daughter. Symptoms include migraine, loss of fine motor control, head banging, violence and aggression, screaming and yelling, loss of rational thought and temporary memory loss, beginning 24 hours after ingestion and gradually diminishing over two weeks. Luckily for us (and by chance) I seldom purchased products containing annatto prior to the challenge, I shudder to think where we would have ended up if she had been consuming annatto regularly all her life. – reader, Qld